Journal: Nature Communications

Article Title: Membrane cholesterol access into a G-protein-coupled receptor

doi: 10.1038/ncomms14505

Figure Lengend Snippet: Control (closed circles) and 5 mM MβCD (40 min) (open circles) treated cells were incubated with different concentrations of [ 3 H]ZM241385 as described in the Methods. These results are mean±s.e.m. values obtained from six separate experiments carried out in duplicate. Kinetic parameters ( B max and K d ) of the corresponding saturation binding curves are indicated at the bottom the figure. ** P <0.01 significantly different from control value according to a Student's t -test.

Article Snippet: The binding data were analysed using Student's t -test, one-way analysis of variance and nonlinear regression fitting to saturation: or competition: binding curves with the GraphPad Prism 5.0 program (GraphPad Software, San Diego, CA, USA).

Techniques: Control, Incubation, Binding Assay

Journal: Bioconjugate chemistry

Article Title: Generation of clickable Pittsburgh Compound B for the detection and capture of β-amyloid in Alzheimer’s Disease brain

doi: 10.1021/acs.bioconjchem.7b00500

Figure Lengend Snippet: (A) A saturation binding assay was used to derive a Kd of 164.7 nM for fluorescent clicked PiB (4). (B) Dose-response curve showing displacement of fluorescent clicked PiB (4) (at 140 nM) with increasing concentrations (0–100,000 nM) of PiB (2) ( ) and clickable PiB (3) ( ). From these data, binding affinities (Ki) of 678.4 and 264.7 nM were determined for compounds 2 and 3, respectively (summarized in Table 1).

Article Snippet: Binding affinities were derived using the saturation binding one-site specific non-linear regression analysis model of GraphPad Prism 5 (GraphPad Software Inc., California).

Techniques: Saturation Assay, Binding Assay

Journal: Biochemical pharmacology

Article Title: Metabolism of bupropion by baboon hepatic and placental microsomes

doi: 10.1016/j.bcp.2011.04.014

Figure Lengend Snippet: A representative saturation curve of OH-BUP formation by baboon hepatic microsomes. The rate of bupropion hydroxylation to OH-BUP was dependent on bupropion concentration and exhibited typical saturation kinetics.

Article Snippet: The V max and apparent K m values were determined using nonlinear regression analysis of the saturation equation (GraphPad Prism 5, Vision 5.01, Graph Pad Software, Inc.).

Techniques: Concentration Assay

Journal: Biochemical pharmacology

Article Title: Metabolism of bupropion by baboon hepatic and placental microsomes

doi: 10.1016/j.bcp.2011.04.014

Figure Lengend Snippet: The representative saturation curve of threohydrobupropion formation by baboon placental microsomes (A) and the effect of chemical inhibitors selective to carbonyl reductases on the formation of threo- and erythrohydrobupropion by baboon placental microsomes (B). The inhibitors of carbonyl reductase are: 4-methylpyrazole (4-MP, 500µM), barbital (BAR, 500µM), flufenamic acid (FLU, 5µM), dicumarol (DIC, 500µM), menadione (MEN, 100µM), and 18β-glycyrrhetinic acid (18β-GA, 0.1µM). The rates of threo-(TB) and erythrohydrobupropion (EB) are expressed as percent of control (absence of inhibitor) and represent the mean ± S.D. of triplicate experiments. ** Statistical significance of p < 0.01 as determined by one-way ANOVA with Tukey’s comparison.

Article Snippet: The V max and apparent K m values were determined using nonlinear regression analysis of the saturation equation (GraphPad Prism 5, Vision 5.01, Graph Pad Software, Inc.).

Techniques: Control, Comparison